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Lactate Dehydrogenase (2H2M) (LDH-3) antibody

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, IF, DB, EIA, ID, IP and RIA. Suitable for Human.
Catalog No. ABIN568287

Quick Overview for Lactate Dehydrogenase (2H2M) (LDH-3) antibody (ABIN568287)

Target

Lactate Dehydrogenase (2H2M) (LDH-3)

Reactivity

Human

Host

  • 3
Rabbit

Clonality

  • 3
Polyclonal

Conjugate

  • 2
  • 1
Un-conjugated

Application

  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
  • Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

    Immunogen

    L-Lactic dehydrogenase, LDH-3(H2M2) isoenzyme, is isolated and purified from Human erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 1.0 mL sterile distilled water.

    Concentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins.

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Lactate Dehydrogenase (2H2M) (LDH-3)

    Alternative Name

    LDH3 H2M2 Isozyme

    Background

    Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.Synonyms: L-Lactic dehydrogenase LDH-3 Isozyme H2M2
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